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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus could contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial role in advertising and marketing cell-cycle development from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been reported but specific nucleotide sequence details is not really however out there.
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Blocking peptides are peptides that bind specifically on the concentrate on antibody and block antibody binding. These peptide generally has the epitope regarded through the antibody.
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This information outlines further doable optimization of mobile and immunoassay parameters to be certain the best possible results are...
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This tutorial outlines even more doable optimization of mobile and immunoassay parameters to be certain the best possible results are...
Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody versus the antibody by itself, you can see which staining is unique.
Goal Details Cyclin E belongs for the very conserved cyclin loved ones, whose members are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation designs which lead to your temporal coordination of each and every mitotic event. Cyclin E sorts a complex with and features as a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates for the G1-S phase boundary which is degraded as cells progress by way of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by equally antibodies...
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